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Response Time of Electrodes Another parameter that is evaluated during the calibration with certain meters that have CAL Check technology is the response time of an electrode. This is evaluated based on the amount of time necessary to reach stability when the electrode is immersed in a new buffer that has a difference in pH larger than 3 pH units from the old one. Offset and Slope of pH Electrode The offset and slope are the most important parameters that can describe the quality of an electrode. With Hanna’s CAL Check technology, the offset of the electrode can be evaluated using one point calibration. Offset is generally determined using a 7.01 pH buffer, however, using CAL Check allows the offset to be based on any calibration point. The acceptable range for offset is ±30 mV although a warning may be displayed. A minimum of two calibration points is necessary to determine the slope. Slope can be evaluated between two calibration points and normally should fall within a range of 92% to 110%, where 100% is 59.16 mV/pH @ 25°C. Calibration Points and pH buffers The calibration of a pH electrode is normally performed using two points: 7 pH, and 4 or 10 pH. This is based on the assumption that the pH electrode is linear from 3 pH up to 10 pH. For the most accurate reading, Hanna recommends using a calibration point closest to the values received during normal measurement. For a variety of applications and measuring points, many Hanna meters offer the ability to calibrate using more than two points. Many Hanna instruments offer 2, 3, or up to 5 calibration points for enhanced accuracy. pH buffers 1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01, and 12.45 cover the entire pH range. During calibration, the recognized pH buffers are temperature compensated by the instrument in order to account for pH variation of buffers due to temperature. For example, a 10.01 pH buffer is 10.01 pH only @ 25°C. A table of temperature variation is printed on the label of each pH buffer. Custom pH Buffers Hanna has implemented the concept of custom pH buffers into many of its instruments. This permits the user to add an industry specific buffer for calibration. However, temperature compensation during calibration is not implemented because the temperature variation correlation is unknown. Stability During Calibration The stability of readings is important in order to avoid incorrect calibration. Based on this, the confirmation of a new calibration point is done only after stability is reached. Users are informed during all processes about the stability conditions, and any instability will restart the stability evaluation. The stability criteria during the calibration is more rigorous than during the measurement. This mode used in Hanna instrumentation avoids errors by confirmation of calibration points during unstable readings. This principle is respected in any type of calibration, manual or automatic. Out of Calibration Range This is an important feature during measurement and is considered Good Laboratory Practice (GLP). The measurement is considered more accurate. If the measurement reading is in a range far from the calibration points, the “out of calibration range” message is displayed. The measured value is shown and the user can accept it, but with the warning from the instrument related to possible inaccuracy. Calibration Reminder The calibration reminder, like “out of calibration range," is a GLP warning message. Regularly scheduled calibrations are crucial for accurate and repeatable measurements. A warning reminder will be displayed when the sensor needs calibration. Measurements can still be used under the warning reminder. Step-by-Step Calibration In order to avoid errors during the calibration procedure, the meters display indicators that can be followed by the user for a successful calibration. If necessary, it is possible for the calibration steps to be performed in a different order by the user. Additional Features GLP and ISO standards require the traceability of operations. Hanna’s GLP document the quality of calibration, plus information to identify the instrument, operator, and the time at which calibration was performed. Logging is a common feature for many instruments and can be used to record readings. Two working modes are available: log-on-demand and automatic or interval logging. With log-on-demand, measurements that are considered important can be saved with the press of the log button. With automatic or interval logging, the instrument saves all the readings according to a specified interval. Another logging mode is Auto-End logging or log on stability. Many Hanna meters include graphic LCD's with features such as tutorials, contextual help, multi-language support, and icons and messages to guide the user through operation and calibration. Introduction 2 pH 2.3 www.hannainst.com | introduction

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